SCIENTIFIC SKILLS

Setting up of screening methodologies of metal-chelating peptides in complex mixture (SPR, SwitchSENSE, MS)

Biocatalysis production of Metal-Chelating Peptides from proteins (enzymatic proteolysis)

Biochemical quantification (OPA, total polyphenols, Flavonoids), Polyphenol extraction, Biochemical antioxidant tests (metal-chelation, reducing power, radical-scavenging activity) and tests in cellulo (coll. CITHEFOR)

Immobilized metal Ion Affinity Chromatography (IMAC) and prediction of IMAC separation (intern LRGP Coll.)

Mass spectrometry (coll. LIBio, L2CM)

Study of molecular interactions, i.e. peptide-metal interactions (SPR, coll. L2CM)

Physical-chemical characterisation of biomolecular assemblies (SLS, DLS, etc.)

Management of students : PhD students, post-dotorates, Master and undergraduated students

Organizing steering committees :

• IMPACT Biomolecules of LUE in the frame of the ISITE project, Representative of the "Metal-chelating peptide" workpackage
• https://lue.univ-lorraine.fr/fr/ressources-naturelles-et-environnement/projet-lue-impact-biomolecules

• MBP'2022, SFGP2017